Strain selection in carbon-limited chemostats affects reproducibility of Thermoanaerobacter ethanolicus fermentations.
نویسندگان
چکیده
منابع مشابه
The mechanism for regulating ethanol fermentation by redox levels in Thermoanaerobacter ethanolicus.
Anaerobes can obtain the entire cell's ATP by glycolysis and remove resulting reducing power by fermentation. There is a delicate balance in redox status to obtain a maximal growth of these cells, and the conditions to change redox fluxes can induce kinds of changes in metabolism. The fundamental knowledge on sensing redox status and coupling redox signals with fermentation pathways is essentia...
متن کاملASYMMETRIC SYNTHESIS OF PHENYL-RING-CONTAINING ALCOHOLS USING THERMOANAEROBACTER ETHANOLICUS W110A SECONDARY ALCOHOL DEHYDROGENASE by MUSA
An enantioselective asymmetric reduction of phenyl ring-containing prochiral ketones toyield the corresponding optically active secondary alcohols was achieved with W110Asecondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus (W110A TeSADH) inTris-HCl buffer using 2-propanol (30%, v/v) as cosolvent and cosubstrate. This concentration of2-propanol was crucial not only...
متن کاملBiophysical and mutagenic analysis of Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase activity and specificity.
The Thermoanaerobacter ethanolicus 39E adhB gene encoding the secondary-alcohol dehydrogenase (secondary ADH) was overexpressed in Escherichia coli at more than 10% of total protein. The recombinant enzyme was purified in high yield (67%) by heat-treatment at 85 degrees C and (NH4)2SO4 precipitation. Site-directed mutants (C37S, H59N, D150N, D150Eand D150C were analysed to test the peptide sequ...
متن کاملA Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase mutant derivative highly active and stereoselective on phenylacetone and benzylacetone.
The secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus 39E (TeSADH) is highly thermostable and solvent-stable, and it is active on a broad range of substrates. These properties make TeSADH an excellent template to engineer an industrial catalyst for chiral chemical synthesis. (S)-1-Phenyl-2-propanol was our target product because it is a precursor to major pharmaceuticals conta...
متن کاملClostridium thermocellum JW20 (ATCC 31549) is a coculture with Thermoanaerobacter ethanolicus.
A PCR assay based on 16S rRNA sequence differences among four thermophilic anaerobic bacterial strains was used to demonstrate contamination of Clostridium thermocellum JW20 (ATCC 31549) with a Thermoanaerobacter ethanolicus strain. Therefore, we suggest that interpretation of experimental results with C. thermocellum JW20 be viewed with caution.
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1992
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.58.2.761-764.1992